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Interferon gamma release upon in vitro restimulation with SARS-CoV-2 Wuhan and Omicron variant antigens. <t>IFNy</t> <t>release</t> was tested in the interferon gamma release assay after overnight stimulation with Omicron Spike variant antigen and Wuhan Spike variant antigen ( x -axis) in individuals with breakthrough infection (BTI, orange circle, n = 14) and non BTI (blue circle, n = 7). A zoomed image (black box) shows the subjects that fell within the regions considered as a negative response (<0.1 IU/mL, shaded red) to SARS-CoV-2, and borderline results (0.1–0.2 IU/mL, shaded orange).
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Interferon gamma release upon in vitro restimulation with SARS-CoV-2 Wuhan and Omicron variant antigens. IFNy release was tested in the interferon gamma release assay after overnight stimulation with Omicron Spike variant antigen and Wuhan Spike variant antigen ( x -axis) in individuals with breakthrough infection (BTI, orange circle, n = 14) and non BTI (blue circle, n = 7). A zoomed image (black box) shows the subjects that fell within the regions considered as a negative response (<0.1 IU/mL, shaded red) to SARS-CoV-2, and borderline results (0.1–0.2 IU/mL, shaded orange).

Journal: Diagnostics

Article Title: Impact of Omicron Variant Infection on Assessment of Spike-Specific Immune Responses Using the EUROIMMUN Quan-T-Cell SARS-CoV-2 Assay and Roche Elecsys Anti-SARS-CoV-2-S

doi: 10.3390/diagnostics13061024

Figure Lengend Snippet: Interferon gamma release upon in vitro restimulation with SARS-CoV-2 Wuhan and Omicron variant antigens. IFNy release was tested in the interferon gamma release assay after overnight stimulation with Omicron Spike variant antigen and Wuhan Spike variant antigen ( x -axis) in individuals with breakthrough infection (BTI, orange circle, n = 14) and non BTI (blue circle, n = 7). A zoomed image (black box) shows the subjects that fell within the regions considered as a negative response (<0.1 IU/mL, shaded red) to SARS-CoV-2, and borderline results (0.1–0.2 IU/mL, shaded orange).

Article Snippet: The Graph visualizing the IFNy release ( ) was created in GraphPad Prism Version 6.0.

Techniques: In Vitro, Variant Assay, Ifn? elispot, Infection